ZB 06 - Nanoelectrochemistry (W.Nogala)

Amperometric biosensors based on alcohol oxidase and peroxidase-like nanozymes for ethanol determination

Author(s): Stasyuk, Nataliya and Demkiv, Olha and Gayda, Galina and Zakalska, Oksana and Nogala, Wojciech and Gonchar, Mykhailo
Title: Amperometric biosensors based on alcohol oxidase and peroxidase-like nanozymes for ethanol determination
Abstract: The aim of the current research is to design alcohol oxidase-based biosensors (ABSs) using hybrid metallic nanoparticles as peroxidases (PO) or PO-like nanozymes (NZs). A lot of PO-like NZs were synthesized and tested with respect to their to substitute natural PO in solution and on amperometric The most effective PO mimetics were coupled with alcohol (AOX) on graphite electrodes (GEs) and characterized. Two types modified GEs, namely, the AOX/nAuCePt/GE and the AOX/nFePtAu/GE show highest sensitivities to ethanol (2600 A.M-1.m(-2) and 1250 respectively), low limits of detection (1.5 mu M and 2.2 mu broad linear ranges (5 - 100 mu M and 12 - 120 mu M), as well as storage stabilities. The most sensitive bioelectrode GE was used as ABS for ethanol determination in real The practical feasibility of the constructed ABS was by determination of ethanol in beverages, human blood and saliva.
Pages: 474
Journal: MICROCHIMICA ACTA
Publisher: SPRINGER
Volume: 189
ID: WOS:000888748700001
Year: 2022
URL: https://doi.org/10.1007/s00604-022-05568-z
DOI: 10.1007/s00604-022-05568-z