ZB 06 - Nanoelectrochemistry (W.Nogala)

Scanning electrochemical microscopy study of laccase within a sol-gel processed silicate film

Author(s): Nogala, Wojciech and Burchardt, Malte and Opallo, Marcin and Rogalski, Jerzy and Wittstock, Gunther
Title: Scanning electrochemical microscopy study of laccase within a sol-gel processed silicate film
Abstract: The enzyme p-diphenol:dioxygen oxidoreductase (laccase, EC 1.10.3.2) was from Cerrena unicolor fungus and embedded in a sol-gel film by acidic condensation of TMOS. The gel was cast to thin films glass. The laccase-containing silicate films were inspected by laser scanning microscopy (CLSM), scanning force microscopy and scanning electrochemical microscopy (SECM). CLSM images in the mode showed aggregates within the silicate films. SECM images the substrate-generation/tip-collection mode using (ABTS) as electron for laccase showed that the position of aggregates coincides with enzymatic activity within the silicate film. The flux from aggregates was detected. SECM images in the redox competition confirmed the assignment and could exclude that topographic observed by CLSM and SFM could be the reason for the image SFM images showed that the aggregates partially dissolve prolonged exposure to aqueous buffer. The experimental setup following one individual aggregate over time with all three techniques which enabled the collection of complementing on morphology and catalytic activity as well as their development over time. (C) 2008 Elsevier B.V. All rights reserved.
Pages: 174-182
Journal: BIOELECTROCHEMISTRY
Publisher: ELSEVIER SCIENCE SA
Addres: PO BOX 564, 1001 LAUSANNE, SWITZERLAND
Volume: 72
ID: ISI:000255504100010
Year: 2008
DOI: 10.1016/j.bioelechem.2008.01.010