ZB 06 - Nanoelectrochemistry (W.Nogala)

Scanning electrochemical microscopy activity mapping of electrodes modified with laccase encapsulated in sol-gel processed matrix

Author(s): Nogala, Wojciech and Szot, Katarzyna and Burchardt, Malte and Martin and Rogalski, Jerzy and Wittstock, Gunther and Opallo, Marcin
Title: Scanning electrochemical microscopy activity mapping of electrodes modified with laccase encapsulated in sol-gel processed matrix
Abstract: Electrodes modified with sol-gel encapsulated laccase (isolated from unicolor) exhibiting mediated or mediatorless dioxygen reduction activity were inspected using laser scanning microscopy, atomic force microscopy and scanning microscopy. Potential-driven leaching of the redox 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) from carbon electrodes covered by hydrophilic silicate-encapsulated laccase detected during electrocatalytic action. Strongly non-homogeneous distribution of the activity towards dioxygen reduction was by redox competition mode of scanning electrochemical microscopy a similar electrode with syringaldazine as redox mediator. peroxide formation at these electrodes is detected at lower than 0.05 V. It is ascribed to the electrochemical reduction at the carbon material while laccase-catalyzed oxygen occurs below 0.35 V without hydrogen peroxide formation. The electrochemical microscopy images of electrodes consisting of carbon nanotubes non-covalently modified with and laccase encapsulated in a sol-gel processed silicate confirm direct electron transfer electrocatalysis in redox mode experiments and show that the enzyme is evenly in the composite film. In conclusion scanning microscopy proved to be useful for mapping of enzyme on different materials. (C) 2010 Elsevier B.V. All rights reserved.
Pages: 101-107
Journal: BIOELECTROCHEMISTRY
Publisher: ELSEVIER SCIENCE SA
Addres: PO BOX 564, 1001 LAUSANNE, SWITZERLAND
Volume: 79
ID: ISI:000278666300017
Year: 2010
DOI: 10.1016/j.bioelechem.2009.12.009