ZB 10 - Soft Condensed Matter (R. Holyst)

Stability of cytoplasmic nanoviscosity during cell cycle of HeLa cells synchronized with Aphidicolin

Author(s): Szczepanski, Krzysztof and Kwapiszewska, Karina and Holyst, Robert
Title: Stability of cytoplasmic nanoviscosity during cell cycle of HeLa cells synchronized with Aphidicolin
Abstract: Nanoviscosity of the cytoplasm is a key factor affecting diffusion of and - as a consequence - rates of biochemical reactions in cell. Nanoviscosity is an outcome of variable chemical and structural which can temporarily change with cell-cycle associated changes intracellular architecture. Thus, the question arises, whether rates biochemical reactions depend on the point of cell cycle. In this we address this topic by constant observation of nanoviscosity of cells cytoplasm during S, G2 and G1 phases after Aphidicolin For this purpose we measured diffusion rates of EGFP using fluorescence correlation spectroscopy (FCS). To our a counter-intuitive stability of cytoplasmic viscosity was during the cell cycle. Our results hint at possible existence robust mechanism maintaining stable physiological viscosity of the cytoplasm, despite huge structural changes during cell cycle.
Journal: SCIENTIFIC REPORTS
Publisher: NATURE PUBLISHING GROUP
Addres: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Volume: 9
ID: ISI:000495611100078
Year: 2019
DOI: 10.1038/s41598-019-52758-6