ZB 06 - Nanoelectrochemistry (W.Nogala)

Bioelectrocatalytic mediatorless dioxygen reduction at carbon ceramic electrodes modified with bilirubin oxidase

Author(s): Nogala, Wojciech and Celebanska, Anna and Szot, Katarzyna and Wittstock, Gunther and Opallo, Marcin
Title: Bioelectrocatalytic mediatorless dioxygen reduction at carbon ceramic electrodes modified with bilirubin oxidase
Abstract: Carbon ceramic electrodes were prepared by sol-gel processing of a precursor - methyltrimethoxysilane (MTMOS) - together with graphite microparticles according to a literature procedure. oxidase (BOx) was adsorbed on this electrode from buffer and this process was followed by atomic force microscopy (AFM). electrodes exhibited efficient mediatorless electrocatalytic towards dioxygen reduction. The activity depends on the time of of the enzyme and the pH. The electrode remains active in solution. The bioelectrocatalytic activity is further increased a fraction of the carbon microparticles is replaced by sulfonated nanoparticles (CNPs). This additive enhances the electrical between the enzyme and the electronic conductor. At pH 7 carbon ceramic electrode modified with bilirubin oxidase retains ca. of its highest activity. The role of the modified nanoparticles is by experiments in which a film embedded in a hydrophobic matrix also exhibited efficient mediatorless biocatalytic reduction. Scanning electrochemical microscopy (SECM) of the electrodes indicated a rather even distribution of the catalytic over the electrode surface. (C) 2010 Elsevier Ltd. All rights reserved.
Pages: 5719-5724
Journal: ELECTROCHIMICA ACTA
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Addres: THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
Volume: 55
ID: ISI:000280422800027
Year: 2010
DOI: 10.1016/j.electacta.2010.05.007