Publication

Nanoelectrodes for determination of reactive oxygen and nitrogen species inside murine macrophages

Author(s): Wang, Yixian and Noel, Jean-Marc and Velmurugan, Jeyavel and Nogala, and Mirkin, Michael V. and Lu, Cong and Collignon, Manon Guille and Lemaitre, Frederic and Amatore, Christian
Title: Nanoelectrodes for determination of reactive oxygen and nitrogen species inside murine macrophages
Abstract: Reactive oxygen and nitrogen species (ROS and RNS) produced by are essential for protecting a human body against bacteria viruses. Micrometer-sized electrodes coated with Pt black have been used for selective and sensitive detection of ROS and in biological systems. To determine ROS and RNS inside macrophages, needs smaller (i.e., nanometer-sized) sensors. In this article, the have been extended to the fabrication and characterization Pt/Pt black nanoelectrodes. Electrodes with the metal surface flush glass insulator, most suitable for quantitative voltammetric were fabricated by electrodeposition of Pt black inside an nanocavity under the atomic force microscope control. Despite a radius, the true surface area of Pt electrodes was large to yield stable and reproducible responses to ROS and in vitro. The prepared nanoprobes were used to penetrate cells and ROS and RNS inside macrophages. Weak and very short leaks of from the vacuoles into the cytoplasm were detected, which a is equipped to clean within a couple of seconds, while higher oxidative bursts due to the emptying of vacuoles outside persist on the time scale of tens of seconds.
Pages: 11534-11539
Journal: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publisher: NATL ACAD SCIENCES
Addres: 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
Volume: 109
ID: ISI:000306837100018
Year: 2012
DOI: 10.1073/pnas.1201552109