ZB 06 - Nanoelectrochemistry (W.Nogala)

Phenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods

Author(s): Matula, Kinga and Richter, Lukasz and Janczuk-Richter, Marta and Nogala, and Grzeszkowiak, Mikolaj and Peplinska, Barbara and Jurga, and Wyroba, Elzbieta and Suski, Szymon and Bilski, Henryk and Adrian and Bluyssen, Hans A. R. and Derebecka, Natalia and Joanna and Los, Joanna M. and Los, Marcin and Decewicz, Przemyslaw and Dziewit, Lukasz and Paczesny, Jan and Holyst, Robert
Title: Phenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods
Abstract: Evolution of bacteria to selective chemical pressure (e.g. antibiotics) well studied in contrast to the influence of physical stressors. Here show that instantaneous physical stress in a homogeneous environment concentration gradient) induces fast adaptation of Escherichia We exposed E. coli to a large number of collisions of around 10(5) bacterium per second with sharp ZnO nanorods. The pressure exerted the bacterial cell wall was up to 10 GPa and induced phenotype The bacteria's shape became more spherical, the density of periplasm increased by around 15\% and the average thickness of cell wall by 30\%. Such E. coli cells appeared almost as bacteria in the standard Gram staining. Additionally, we a combination of changes occurring at the genomic level identified in form of single nucleotide polymorphisms) and of expression of 61 genes encoding proteins involved in of fatty acids, glycolysis, the citric acid cycle, as as uptake of amino acids and enzyme cofactors. Thus, we show that undergo phenotypic changes upon instantaneous, acute physical stress without any obviously available time for gradual adaptation.
Journal: SCIENTIFIC REPORTS
Publisher: NATURE PUBLISHING GROUP
Addres: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
Volume: 9
ID: ISI:000471216400004
Year: 2019
DOI: 10.1038/s41598-019-44727-w