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ZB 10 - Soft Condensed Matter (R. Holyst)
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!

Publication

Cell extract gels as an example of active matter

Author(s): Wisniewska, Agnieszka and Kalwarczyk, Tomasz and Szymanski, Jedrzej and Kryszczuk, Katarzyna and Matula, Kinga and Holyst, Robert
Title: Cell extract gels as an example of active matter
Abstract: Cell lysates (cellular extracts) constitute a perfect imitation of the environment that can provide insight into cellular to external stimuli. However, most of the presented results are for diluted lysates that do not reflect the actual properties a crowded cellular environment. Here, we report for the first time measurement of the viscosity and shear storage modulus of highly Escherichia coli(E. coli) lysates with and without triphosphate (ATP). By cleavage of DNA content, we showed the of shear storage modulus G decreases by 19-31\% in comparison to samples. The addition of molecules that provides energy (ATP) to rebuild the structure of the lysate by reversibly increasing properties over elastic ones. When the energy delivered in the of ATP is consumed by the unliving bacterial lysate, the system returns to its initial state.
Pages: 575-582
Journal: RHEOLOGICA ACTA
Publisher: SPRINGER
Addres: ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES
Volume: 59
ID: ISI:000541397200001
Year: 2020
DOI: 10.1007/s00397-020-01213-9

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