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ZB 10 - Soft Condensed Matter (R. Holyst)

We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!
We are different, but we all do great Science; and we have a lot of fun doing it!

Publication

Influence of nano-viscosity and depletion interactions on cleavage of by enzymes in glycerol and poly(ethylene glycol) solutions: qualitative analysis

Author(s): Hou, Sen and Ziebacz, Natalia and Kalwarczyk, Tomasz and Kaminski, Tomasz S. and Wieczorek, Stefan A. and Holyst, Robert
Title: Influence of nano-viscosity and depletion interactions on cleavage of by enzymes in glycerol and poly(ethylene glycol) solutions: qualitative analysis
Abstract: Biochemical reactions in living systems take place in an environment by various macromolecules and ligands. Therefore experimental obtained in buffer do not reflect in vivo conditions. We have used poly(ethylene glycol) (PEG) 6000 and PEG 8 M solutions to the influence of the crowded environment on cleavage of DNA by restriction enzyme HindIII. PEG 6000 solution can slow down the cleavage process. However, neither PEG 8 M of the same viscosity as PEG 6000 solution nor glycerol of the same concentration as PEG 6000 solution slows the of DNA appreciably. The viscosity experienced by the (here called nano-viscosity) and aggregation induced by the interactions between DNA molecules in polymer solution (PEG are two factors responsible for slow cleavage of DNA. We have out the change of pH and denaturation of HindIII as possible sources for the effect.
Pages: 3092-3099
Journal: SOFT MATTER
Volume: 7
ID: ISI:000288677200008
Year: 2011
DOI: 10.1039/c0sm00899k